Resistance, biguanide sorption and biguanide-induced pentose leakage during encystment of Acanthamoeba castellanii
نویسندگان
چکیده
منابع مشابه
Phagocytosis affects biguanide sensitivity of Acanthamoeba spp.
The incidence of Acanthamoeba keratitis, a disease associated with contact lens wear, has been in apparent decline with the advent of multipurpose contact lens solutions. The concentrations of the biguanides chlorhexidine digluconate (CHX) and particularly polyhexamethylene biguanide (PHMB) included in multipurpose solutions (MPSs) are sublethal for amoebae. We evaluated by flow cytometry the e...
متن کاملDouble-Biguanide Therapy for Resistant Acanthamoeba Keratitis
AIMS To report the clinical and diagnostic findings of a patient with Acanthamoeba keratitis resistant to both polyhexamethylene biguanide (PHMB)-hexamidine and chlorhexidine-hexamidine treatment. METHODS Slit-lamp biomicroscopy, corneal cell scraping and histopathology were performed on a 39-year-old woman presenting with corneal ulcer in her left eye. RESULTS The patient was successfully ...
متن کاملInduced encystment improves resistance to preservation and storage of Acanthamoeba castellanii.
Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at -70 degrees C using dimethyl sulfoxide (DMSO; 5 or 10%) as cryopreservant. As A. castellanii are naturally capable...
متن کاملDNA synthesis in growth and encystment of Acanthamoeba castellanii.
Differentiation of Acanthamoeba castellanii into dormant cysts occurs spontaneously in stationary phase cultures, or can be induced experimentally by starvation. Although no further increase in cell density occurred after induction in either case, incorporation of [H]thymidine into DNA continued at a reduced rate through the period when differentiated products (cyst wall components) were formed...
متن کاملChanges in transfer ribonucleic acids accompanying encystment in Acanthamoeba castellanii.
Transfer ribonucleic acid (tRNA) from exponentially growing cells (trophozoites) and from precysts of Acanthamoeba castellanii were examined by reversed-phase column (RPC-2) chromatography. This system gave excellent resolution of isoaccepting species of tRNA. The tRNAs for 12 amino acids were studied. A comparison of trophozoite and precyst tRNA elution profiles revealed no apparent difference...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2004
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2004.02260.x